WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H577717
CAS#: 438-67-5 (sodium)
Description: Estrone sulfate, also known as E1S, E1-S, E1SO4 and estrone 3-sulfate, is a natural, endogenous steroid and an estrogen ester and conjugate. Estrone sulfate is the most abundant circulating estrogen and it has the potential to be used as a biomarker in certain conditions where estimation of low levels of estrogen or changes in relative levels of estrogens are important. E1-S showed lower levels in malignant tissues of breast cancer and endometrial cancer. Increased sulfatase activity in the malignant tissue hydrolyzes E1-S to E1, which may develop the tumors.
Hodoodo Cat#: H577717
Name: Estrone sulfate sodium
CAS#: 438-67-5 (sodium)
Chemical Formula: C18H21NaO5S
Exact Mass: 372.10
Molecular Weight: 372.410
Elemental Analysis: C, 58.05; H, 5.68; Na, 6.17; O, 21.48; S, 8.61
Related CAS #: 481-97-0 (free acid) 438-67-5 (sodium)
Synonym: Estrone sulfate sodium salt; Conestoral; Estrone sodium sulfate; Estrone sulfate sodium; Morestin; NSC 18313; Sodium estrone sulfate; Sodium estrone-3-sulfate;
IUPAC/Chemical Name: 17-Oxoestra-1,3,5(10)-trien-3-yl sodium sulfate
InChi Key: VUCAHVBMSFIGAI-ZFINNJDLSA-M
InChi Code: InChI=1S/C18H22O5S.Na/c1-18-9-8-14-13-5-3-12(23-24(20,21)22)10-11(13)2-4-15(14)16(18)6-7-17(18)19;/h3,5,10,14-16H,2,4,6-9H2,1H3,(H,20,21,22);/q;+1/p-1/t14-,15-,16+,18+;/m1./s1
SMILES Code: O=S(OC(C=C1)=CC(CC[C@@]2([H])[C@]3([H])CC4)=C1[C@@]2([H])CC[C@]3(C)C4=O)([O-])=O.[Na+]
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: To be determined
Shelf Life: >2 years if stored properly
Drug Formulation: To be determined
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | |
| In vitro activity: | |
| In vivo activity: |
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 30.0 | 80.56 | |
| DMSO | 30.0 | 80.56 | |
| Ethanol | 0.5 | 1.34 | |
| DMSO:PBS (pH: 7.2) (1:1) | 2.0 | 5.37 |
The following data is based on the product molecular weight 372.41 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Marroquín-Flores RA, Paitz RT, Bowden RM. Temperature fluctuations and estrone sulfate affect gene expression via different mechanisms to promote female development in a species with temperature-dependent sex determination. J Exp Biol. 2022 Aug 15;225(16):jeb244211. doi: 10.1242/jeb.244211. Epub 2022 Aug 16. PMID: 35860927.
2: Ledeck J, Dufour P, Evrard É, Le Goff C, Peeters S, Brutinel F, Egyptien S, Deleuze S, Cavalier É, Ponthier J. Evolution of 17-β-estradiol, estrone and estrone-sulfate concentrations in late pregnancy of different breeds of mares using Liquid Chromatography and Mass Spectrometry. Theriogenology. 2022 Sep 1;189:86-91. doi: 10.1016/j.theriogenology.2022.06.004. Epub 2022 Jun 11. PMID: 35738034.
3: Frisée V, Rigaux G, Dufour P, Barbato O, Brutinel F, Egyptien S, Bossaert P, Deleuze S, Cavalier E, Ponthier J. American Bison (Bison bison) reproductive endocrinology: serum Pregnancy Associated Glycoproteins (PAG), Progesterone, Estrone and Estrone-Sulfate in non pregnant animals and during gestation. Domest Anim Endocrinol. 2022 Jan;78:106684. doi: 10.1016/j.domaniend.2021.106684. Epub 2021 Sep 16. PMID: 34634728.
4: Müller JP, Keufgens L, Gründemann D. Hyperosmolarity stimulates transporter- mediated insertion of estrone sulfate into the plasma membrane, but inhibits the uptake by SLC10A1 (NTCP). Biochem Pharmacol. 2021 Apr;186:114484. doi: 10.1016/j.bcp.2021.114484. Epub 2021 Feb 20. PMID: 33617845.
5: Esteller-Vico A, Ball BA, Bridges JW, Hughes SE, Squires EL, Troedsson MHT. Changes in circulating concentrations of testosterone and estrone sulfate after human chorionic gonadotropin administration and subsequent to castration of 2-year-old stallions. Anim Reprod Sci. 2021 Feb;225:106670. doi: 10.1016/j.anireprosci.2020.106670. Epub 2020 Dec 3. PMID: 33338983.
6: Ankarberg-Lindgren C, Andersson MX, Dahlgren J. Determination of estrone sulfate, testosterone, androstenedione, DHEAS, cortisol, cortisone, and 17α-hydroxyprogesterone by LC-MS/MS in children and adolescents. Scand J Clin Lab Invest. 2020 Dec;80(8):672-680. doi: 10.1080/00365513.2020.1829699. Epub 2020 Oct 14. PMID: 33258387.
7: Liu X, Schwarz T, Murawski M, Tayade C, Kridli R, Prieto Granados AM, Sharma C, Bartlewski PM. Measurements of circulating progesterone and estrone sulfate concentrations as a diagnostic and prognostic tool in porcine pregnancy revisited. Domest Anim Endocrinol. 2020 Apr;71:106402. doi: 10.1016/j.domaniend.2019.106402. Epub 2019 Dec 2. PMID: 31972516.
8: Paitz RT, Angles R, Cagney E. In ovo metabolism of estradiol to estrone sulfate in chicken eggs: Implications for how yolk estradiol influences embryonic development. Gen Comp Endocrinol. 2020 Feb 1;287:113320. doi: 10.1016/j.ygcen.2019.113320. Epub 2019 Nov 9. PMID: 31715137.
9: Schuler G, Bernhardt-Welte AW, Failing K, Hoffmann B. Jahreszeitlicher Konzentrationsverlauf von Testosteron, Estron und Estronsulfat im peripheren Blut von Eselhengsten [Concentrations of testosterone, estrone and estrone sulfate in peripheral blood of donkey stallions in relation to season]. Tierarztl Prax Ausg G Grosstiere Nutztiere. 2019 Oct;47(5):294-297. German. doi: 10.1055/a-0988-0964. Epub 2019 Oct 21. PMID: 31634940.
10: Singh SP, Ramachandran N, Sharma N, Goel AK, Gururaj K, Kharche SD. Temporal changes in plasma profile of pregnancy-associated glycoprotein, progesterone and estrone sulfate associated with fetal number during early- and mid-pregnancy in goats. Anim Reprod Sci. 2019 Jun;205:115-125. doi: 10.1016/j.anireprosci.2019.04.010. Epub 2019 Apr 24. PMID: 31040054.
11: Gilligan LC, Gondal A, Tang V, Hussain MT, Arvaniti A, Hewitt AM, Foster PA. Estrone Sulfate Transport and Steroid Sulfatase Activity in Colorectal Cancer: Implications for Hormone Replacement Therapy. Front Pharmacol. 2017 Mar 7;8:103. doi: 10.3389/fphar.2017.00103. PMID: 28326039; PMCID: PMC5339229.
12: Skwara P, Schömig E, Gründemann D. A novel mode of operation of SLC22A11: Membrane insertion of estrone sulfate versus translocation of uric acid and glutamate. Biochem Pharmacol. 2017 Mar 15;128:74-82. doi: 10.1016/j.bcp.2016.12.020. Epub 2016 Dec 25. PMID: 28027879.
13: Rezvanpour A, Don-Wauchope AC. Clinical implications of estrone sulfate measurement in laboratory medicine. Crit Rev Clin Lab Sci. 2017 Mar;54(2):73-86. doi: 10.1080/10408363.2016.1252310. Epub 2016 Dec 14. PMID: 27960570.
14: Higuchi T, Endo M, Hanamura T, Gohno T, Niwa T, Yamaguchi Y, Horiguchi J, Hayashi S. Contribution of Estrone Sulfate to Cell Proliferation in Aromatase Inhibitor (AI) -Resistant, Hormone Receptor-Positive Breast Cancer. PLoS One. 2016 May 26;11(5):e0155844. doi: 10.1371/journal.pone.0155844. PMID: 27228187; PMCID: PMC4882040.
15: Bjerregaard-Olesen C, Ghisari M, Kjeldsen LS, Wielsøe M, Bonefeld-Jørgensen EC. Estrone sulfate and dehydroepiandrosterone sulfate: Transactivation of the estrogen and androgen receptor. Steroids. 2016 Jan;105:50-8. doi: 10.1016/j.steroids.2015.11.009. Epub 2015 Dec 5. PMID: 26666359.
16: Vincze B, Kapuvári B, Udvarhelyi N, Horváth Z, Mátrai Z, Czeyda-Pommersheim F, Kőhalmy K, Kovács J, Boldizsár M, Láng I, Kásler M. Serum estrone concentration, estrone sulfate/estrone ratio and BMI are associated with human epidermal growth factor receptor 2 and progesterone receptor status in postmenopausal primary breast cancer patients suffering invasive ductal carcinoma. Springerplus. 2015 Jul 31;4:387. doi: 10.1186/s40064-015-1171-8. PMID: 26240785; PMCID: PMC4520825.
17: Giton F, Sirab N, Franck G, Gervais M, Schmidlin F, Ali T, Allory Y, de la Taille A, Vacherot F, Loric S, Fiet J. Evidence of estrone-sulfate uptake modification in young and middle-aged rat prostate. J Steroid Biochem Mol Biol. 2015 Aug;152:89-100. doi: 10.1016/j.jsbmb.2015.05.002. Epub 2015 May 6. PMID: 25958048.
18: Hevir-Kene N, Rižner TL. The endometrial cancer cell lines Ishikawa and HEC-1A, and the control cell line HIEEC, differ in expression of estrogen biosynthetic and metabolic genes, and in androstenedione and estrone-sulfate metabolism. Chem Biol Interact. 2015 Jun 5;234:309-19. doi: 10.1016/j.cbi.2014.11.015. Epub 2014 Nov 28. PMID: 25437045.
19: Abeni F, Capelletti M, Terzano GM, Federici C, Speroni M, Petrera F, Dal Prà A, Galli C, Duchi R, Lazzari G, Pirlo G, Aleandri R. Plasma estrone sulfate, clinical biochemistry, and milk yield of dairy cows carrying a fetus from a bull or its clone. Theriogenology. 2014 Oct 15;82(7):972-81. doi: 10.1016/j.theriogenology.2014.07.015. Epub 2014 Jul 23. PMID: 25139753.
20: Hoffmann B, Bernhardt AW, Failing K, Schuler G. Profile von Estron, Estronsulfat und Progesteron während der Trächtigkeit beim Esel (Equus asinus) [Profiles of estrone, estrone sulfate and progesterone in donkey (Equus asinus) mares during pregnancy]. Tierarztl Prax Ausg G Grosstiere Nutztiere. 2014;42(1):32-9. German. PMID: 24518882.
