WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H318358
CAS#: 51-60-5 (methylsulfate)
Description: Neostigmine is a parasympathomimetic compound that acts as a reversible acetylcholinesterase inhibitor. Given that it is a quaternary amine (unlike physostigmine, which is a tertiary amine), it penetrates the central nervous system poorly and has lower propensity to cause side effects there.
Hodoodo Cat#: H318358
Name: Neostigmine methylsulfate
CAS#: 51-60-5 (methylsulfate)
Chemical Formula: C13H22N2O6S
Exact Mass: 223.14
Molecular Weight: 334.387
Elemental Analysis: C, 46.70; H, 6.63; N, 8.38; O, 28.71; S, 9.59
Related CAS #: 59-99-4 (cation) 114-80-7 (bromide) 51-60-5 (methylsulfate) 1212-37-9 (iodide) 588-17-0 (hydroxide)
Synonym: Neostigmine, Prostigmin, Vagostigmin, Polstigmine; Neostigmine methylsulfate
IUPAC/Chemical Name: 3-((dimethylcarbamoyl)oxy)-N,N,N-trimethylbenzenaminium methyl sulfate
InChi Key: OSZNNLWOYWAHSS-UHFFFAOYSA-M
InChi Code: InChI=1S/C12H19N2O2.CH4O4S/c1-13(2)12(15)16-11-8-6-7-10(9-11)14(3,4)5;1-5-6(2,3)4/h6-9H,1-5H3;1H3,(H,2,3,4)/q+1;/p-1
SMILES Code: C[N+](C)(C1=CC=CC(OC(N(C)C)=O)=C1)C.O=S(OC)([O-])=O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO, not in water
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
Biological target: | |
In vitro activity: | |
In vivo activity: |
The following data is based on the product molecular weight 334.39 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1: Abad-Gurumeta A, Ripollés-Melchor J, Casans-Francés R, Espinosa A, Martínez-Hurtado E, Fernández-Pérez C, Ramírez JM, López-Timoneda F, Calvo-Vecino JM; Evidence Anaesthesia Review Group. A systematic review of sugammadex vs neostigmine for reversal of neuromuscular blockade. Anaesthesia. 2015 Dec;70(12):1441-52. doi: 10.1111/anae.13277. Review. PubMed PMID: 26558858.
2: Lauretti GR. The evolution of spinal/epidural neostigmine in clinical application: Thoughts after two decades. Saudi J Anaesth. 2015 Jan;9(1):71-81. doi: 10.4103/1658-354X.146319. Review. PubMed PMID: 25558203; PubMed Central PMCID: PMC4279354.
3: Liu SH, Tseng CY, Lin CC. Is neostigmine effective in severe pufferfish-associated tetrodotoxin poisoning? Clin Toxicol (Phila). 2015 Jan;53(1):13-21. doi: 10.3109/15563650.2014.980581. Epub 2014 Nov 20. Review. PubMed PMID: 25410493.
4: Valle RG, Godoy FL. Neostigmine for acute colonic pseudo-obstruction: A meta-analysis. Ann Med Surg (Lond). 2014 Jun 19;3(3):60-4. doi: 10.1016/j.amsu.2014.04.002. eCollection 2014 Sep. Review. PubMed PMID: 25568788; PubMed Central PMCID: PMC4284455.
5: Yang L, Yang D, Li Q, Zuo Y, Lu D. Neostigmine for reversal of neuromuscular block in paediatric patients. Cochrane Database Syst Rev. 2014 May 5;5:CD010110. doi: 10.1002/14651858.CD010110.pub2. Review. PubMed PMID: 24793636.
6: Elsner JL, Smith JM, Ensor CR. Intravenous neostigmine for postoperative acute colonic pseudo-obstruction. Ann Pharmacother. 2012 Mar;46(3):430-5. doi: 10.1345/aph.1Q515. Epub 2012 Feb 28. Review. PubMed PMID: 22388328.
7: Kayani B, Spalding DR, Jiao LR, Habib NA, Zacharakis E. Does neostigmine improve time to resolution of symptoms in acute colonic pseudo-obstruction? Int J Surg. 2012;10(9):453-7. doi: 10.1016/j.ijsu.2012.08.008. Epub 2012 Sep 14. Review. PubMed PMID: 22985773.
8: Paton F, Paulden M, Chambers D, Heirs M, Duffy S, Hunter JM, Sculpher M, Woolacott N. Sugammadex compared with neostigmine/glycopyrrolate for routine reversal of neuromuscular block: a systematic review and economic evaluation. Br J Anaesth. 2010 Nov;105(5):558-67. doi: 10.1093/bja/aeq269. Epub 2010 Oct 8. Review. PubMed PMID: 20935005; PubMed Central PMCID: PMC2955536.
9: Mizikov VM, Stamov VI, Deshko IuV, Ialich AIu. [Problem of neuromuscular block reversion: neostigmine and sugammadex]. Anesteziol Reanimatol. 2010 Mar-Apr;(2):40-3. Review. Russian. PubMed PMID: 20524330.
10: Lee C, Katz RL. Clinical implications of new neuromuscular concepts and agents: so long, neostigmine! So long, sux! J Crit Care. 2009 Mar;24(1):43-9. doi: 10.1016/j.jcrc.2008.08.009. Epub 2009 Jan 17. Review. PubMed PMID: 19272538.
11: McNamara R, Mihalakis MJ. Acute colonic pseudo-obstruction: rapid correction with neostigmine in the emergency department. J Emerg Med. 2008 Aug;35(2):167-70. doi: 10.1016/j.jemermed.2007.06.043. Epub 2008 Feb 1. Review. PubMed PMID: 18242923.
12: Ho KM, Ismail H, Lee KC, Branch R. Use of intrathecal neostigmine as an adjunct to other spinal medications in perioperative and peripartum analgesia: a meta-analysis. Anaesth Intensive Care. 2005 Feb;33(1):41-53. Review. PubMed PMID: 15957690.
13: Maeda N, Murai M, Nakazawa A, Suzuki O, Mizuno Y, Yamamoto Y, Shimizu K, Hibi T. [A case of chronic colonic pseudo-obstruction due to amyloidosis successfully treated with neostigmine]. Nihon Shokakibyo Gakkai Zasshi. 2004 Jun;101(6):609-15. Review. Japanese. PubMed PMID: 15233262.
14: Habib AS, Gan TJ. Use of neostigmine in the management of acute postoperative pain and labour pain: a review. CNS Drugs. 2006;20(10):821-39. Review. PubMed PMID: 16999453.
15: Roelants F. The use of neuraxial adjuvant drugs (neostigmine, clonidine) in obstetrics. Curr Opin Anaesthesiol. 2006 Jun;19(3):233-7. Review. PubMed PMID: 16735803.
16: Eisenach JC. Three novel spinal analgesics: clonidine, neostigmine, amitriptyline. Reg Anesth. 1996 Nov-Dec;21(6 Suppl):81-3. Review. PubMed PMID: 8956428.
17: Hazama M, Nakano M, Shinozaki M, Fujisawa M, Okamoto Y, Ren KZ, Oka N, Hamaguchi T, Okada H, Matsumoto O, et al. [A case of a complete paraplegic fathering a girl following artificial insemination after an intrathecal neostigmine injection]. Hinyokika Kiyo. 1988 Jun;34(6):1047-50. Review. Japanese. PubMed PMID: 3066212.