WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H524767
CAS#: 142715-48-8(HCl)
Description: CP 100356 is a high affinity P-glycoprotein (P-gp) inhibitor (Ki values are 58 and 94 nM for mouse Pgp1a and Pgp1b isoforms). Inhibits calcein-AM uptake in MDR1-transfected MDCKII cells (IC50 = 0.5 μM) and prazosin transport in BCRP-transfected MDCKII cells (IC50 = 1.5 μM). CP 100356 modulates multidrug resistance.
Hodoodo Cat#: H524767
Name: CP-100356 HCl
CAS#: 142715-48-8(HCl)
Chemical Formula: C31H37ClN4O6
Exact Mass: 560.26
Molecular Weight: 597.109
Elemental Analysis: C, 62.36; H, 6.25; Cl, 5.94; N, 9.38; O, 16.08
Related CAS #: 142716-85-6 (free base) 142715-48-8(HCl)
Synonym: CP 100356; CP100356; CP-100356; CP-100356 hydrochloride, CP-100356 HCl.
IUPAC/Chemical Name: 4-(6,7-dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl)-N-(3,4-dimethoxyphenethyl)-6,7-dimethoxyquinazolin-2-amine hydrochloride
InChi Key: WWCHXVYTCMPAMV-UHFFFAOYSA-N
InChi Code: InChI=1S/C31H36N4O6.ClH/c1-36-24-8-7-19(13-25(24)37-2)9-11-32-31-33-23-17-29(41-6)28(40-5)16-22(23)30(34-31)35-12-10-20-14-26(38-3)27(39-4)15-21(20)18-35;/h7-8,13-17H,9-12,18H2,1-6H3,(H,32,33,34);1H
SMILES Code: COC1=CC2=NC(NCCC3=CC=C(OC)C(OC)=C3)=NC(N4CC5=C(C=C(OC)C(OC)=C5)CC4)=C2C=C1OC.[H]Cl
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO, not in water
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
Biological target: | |
In vitro activity: | |
In vivo activity: |
Solvent | Max Conc. mg/mL | Max Conc. mM | |
---|---|---|---|
Solubility | |||
Soluble in DMSO, not in water | 0.0 | 100.00 |
The following data is based on the product molecular weight 597.11 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1: Li M, de Graaf IA, de Jager MH, Groothuis GM. Rat precision-cut intestinal slices to study P-gp activity and the potency of its inhibitors ex vivo. Toxicol In Vitro. 2015 Aug;29(5):1070-8. doi: 10.1016/j.tiv.2015.04.011. Epub 2015 Apr 25. PubMed PMID: 25917215.
2: Kalgutkar AS, Frederick KS, Chupka J, Feng B, Kempshall S, Mireles RJ, Fenner KS, Troutman MD. N-(3,4-dimethoxyphenethyl)-4-(6,7-dimethoxy-3,4-dihydroisoquinolin-2[1H]-yl)-6,7- dimethoxyquinazolin-2-amine (CP-100,356) as a "chemical knock-out equivalent" to assess the impact of efflux transporters on oral drug absorption in the rat. J Pharm Sci. 2009 Dec;98(12):4914-27. doi: 10.1002/jps.21756. PubMed PMID: 19373887.
3: Zhao P, Kunze KL, Lee CA. Evaluation of time-dependent inactivation of CYP3A in cryopreserved human hepatocytes. Drug Metab Dispos. 2005 Jun;33(6):853-61. Epub 2005 Mar 2. PubMed PMID: 15743977.
4: van Veen HW, Margolles A, Müller M, Higgins CF, Konings WN. The homodimeric ATP-binding cassette transporter LmrA mediates multidrug transport by an alternating two-site (two-cylinder engine) mechanism. EMBO J. 2000 Jun 1;19(11):2503-14. PubMed PMID: 10835349; PubMed Central PMCID: PMC212756.
5: Taylor JC, Ferry DR, Higgins CF, Callaghan R. The equilibrium and kinetic drug binding properties of the mouse P-gp1a and P-gp1b P-glycoproteins are similar. Br J Cancer. 1999 Nov;81(5):783-9. PubMed PMID: 10555746; PubMed Central PMCID: PMC2374293.
6: Kajiji S, Dreslin JA, Grizzuti K, Gros P. Structurally distinct MDR modulators show specific patterns of reversal against P-glycoproteins bearing unique mutations at serine939/941. Biochemistry. 1994 May 3;33(17):5041-8. PubMed PMID: 8172879.