WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H575808
CAS#: 56455-90-4 (HCl)
Description: Dicarboxidine is a chromogen for the detection of barbiturates, meprobamate and some other sedatives on thin-layer chromatograms. Dicarboxidine is a safe substitute for conventional benzidine type reagents. Dicarboxidine can be used for detection of N-Boc-protected amino acids and peptides by TLC. Dicarboxidine is a substrate for peroxidases as horse radish peroxidase.
Hodoodo Cat#: H575808
Name: Dicarboxidine HCl
CAS#: 56455-90-4 (HCl)
Chemical Formula: C20H26Cl2N2O6
Exact Mass: 460.12
Molecular Weight: 461.340
Elemental Analysis: C, 52.07; H, 5.68; Cl, 15.37; N, 6.07; O, 20.81
Related CAS #: 56455-90-4 (HCl) 34915-18-9 (free base)
Synonym: Dicarboxidine dihydrochloride; Dicarboxidine HCl,
IUPAC/Chemical Name: Butanoic acid, 4,4'-((4,4'-diamino-(1,1'-biphenyl)-3,3'-diyl)bis(oxy))bis-, dihydrochloride
InChi Key: BABQSVIBQAEEQX-UHFFFAOYSA-N
InChi Code: InChI=1S/C20H24N2O6.2ClH/c21-15-7-5-13(11-17(15)27-9-1-3-19(23)24)14-6-8-16(22)18(12-14)28-10-2-4-20(25)26;;/h5-8,11-12H,1-4,9-10,21-22H2,(H,23,24)(H,25,26);2*1H
SMILES Code: NC1=CC=C(C2=CC=C(N)C(OCCCC(O)=O)=C2)C=C1OCCCC(O)=O.[H]Cl.[H]Cl
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
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The following data is based on the product molecular weight 461.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
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| In vivo protocol: |
1: Chen R, Li TM, Merrick H, Parrish RF, Bruno V, Kwong A, Stiso C, Litman DJ. An internal clock reaction used in a one-step enzyme immunochromatographic assay of theophylline in whole blood. Clin Chem. 1987 Sep;33(9):1521-5. PubMed PMID: 3304712.
2: Paul KG, Ohlsson PI, Jönsson NA. The assay of peroxidases by means of dicarboxidine on enzyme-linked immunosorbent assay level. Anal Biochem. 1982 Jul 15;124(1):102-7. PubMed PMID: 7125217.
3: Swolin B, Roberts D, Waldenström J. Quantitative determination of plasma hemoglobin using dicarboxidine. Clin Chim Acta. 1982 Jun 3;121(3):389-91. PubMed PMID: 7105402.
4: Svahn CM, Gyllander J. Dicarboxidine [gamma, gamma'-(4,4'-diamino-3,3'-biphenylylenedioxy)dibutyric acid] dihydrochloride as a chromogen for the detection of barbiturates, meprobamate and some other sedatives on thin-layer chromatograms. J Chromatogr. 1979 Feb 11;170(1):294-5. PubMed PMID: 544628.
5: Jönsson NA, Gröningsson K, Pavlu B, Vessman J, Westlund LE. gamma,gamma'-(4,4'-Diamino-3,3'-biphenylylenedioxy)dibutyric acid (dicarboxidine) a safer substitute for benzidine type chromogens. Arzneimittelforschung. 1979;29(2):187-93. PubMed PMID: 582129.
6: Pliss GB, Vol'fson NI. [Possible carcinogenic action of chromogen dicarboxidine]. Vopr Onkol. 1979;25(7):43-8. Russian. PubMed PMID: 483680.
